Allegro™ XRS 25 Bioreactor System

Unique bi-axial agitation improves cell culture performance

Description

Unique bi-axial agitation improves cell culture performance

The Allegro XRS 25 bioreactor system is a single-use bioreactor system with unique agitation and control properties designed for the cultivation of mammalian cells in suspension culture under controlled conditions. It is suitable for applications ranging from general life sciences research to seed train operations and full cGMP production at the 2 to 25 L scale.

Because of its unique bi-axial agitation and the subsequent shorter mixing times and higher mass transfer properties, the Allegro XRS 25 bioreactor system produces superior performance compared to competitor rocker systems, as measured by higher density, better cell viability, and higher expression levels.

The Allegro XRS 25 bioreactor system rocks on two axes simultaneously (bi-axial agitation), producing a low turbulence, swirling pattern within the 3-dimensional XRS 25 biocontainer bag. Under similar agitation conditions, the Allegro XRS 25 bioreactor system mixes almost 3 times faster than conventional rockers (Table 1), and demonstrates much higher mass transfer properties as measured by kLa values (Table 2).

Specification

General System Specifications

Dimensions
(W x D x H)

Controller
Platform

600 mm x 450 mm x 230 mm
664 mm x 379 mm x 331 mm (allow for 200 mm lid overhang at the rear of the platform, and factor in an additional 370 mm for height when the lid is open)

Weight

Controller
Platform 78 kg (104 kg filled) (with filled biocontainer bag)

20 kg
78 kg (104 kg filled)

Gases

Gas connections
Gas flow control
TMFC pressurized air
TMFC oxygen
TMFC carbon dioxide
TMFC nitrogen

Inlet and outlet quick connectors 6 mm OD
TMFC
0 - 1000 mL/min
0 - 1000 mL/min
0 - 1000 mL/min
0 - 1000 mL/min

Pumps

Quantity and connection

3 x variable speed peristaltic pumps, 0 - 211 rpm, maximum tubing OD 8 mm

Aeration

Inlet filter

0.2 μm single-use filter

Gas delivery
Exhaust filter
Exhaust heater

Gas mix injected into biocontainer bag headspace
0.2 μm single-use filter
Silicone heater mat

Bi-Axial Agitation

Speed

1 - 35 rpm

Angle
Control

-15 to +15 degrees in both X and Y directions
Stepper motor controlled

Process

Working volumes
Temperature control
Temperature range
pH control
Dissolved oxygen control

2 - 25 liters
Heating blanket, ambient convective cooling
10 - 40 °C
6 - 8, integrated single-use optical sensor
0 - 100%, integrated single-use optical sensor

The Allegro XRS 25 bioreactor system is available as a standard product; however, both the bioreactor hardware and consumables can be customized. Our teams are specialized in upstream and downstream processing and will gladly help find the right technology for any part of your manufacturing process. It is possible to combine the Allegro products to meet any requirement in the upstream part of the process:

Media preparation
Buffer preparation
Media sterilization and aseptic transfer
Liquid transfer into the bioreactor (e.g. glucose, anti-foam, base, etc.)
Seed train solutions
Cell harvest and separation
Perfusion modules

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Point of Product

Description

Specification

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