96-Channel Ultra High Throughput Octet System: Octet RH96 System

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Bio-Layer Interferometry (BLI) based on fiber optic biosensors
Sample volume
40–80 μL/well (384-well tilted bottom microplate) and 180–220 μL/well (96-well microplate), nondestructive testing, easily recovered
Data presentation
Sensorgrams displaying kinetic traces or concentration binding rates Epitope binning and cross-blocking matrices and trace overlays Tabulated kinetic or concentration data
Robot compatible microplate and biosensor tray loading
Orbital flow capacity
Static or 100–1500 rpm
Analysis temperature
Ambient + 4°C to 40°C, in 1°C increments
Association rate constant (ka)
101 to 107 M-1 s-1
Dissociation rate constant (kd)
10-6 to 10-1 s-1
Affinity constant (KD)
1 mM – 10 pM
Baseline noise (RMS)
<3 pm (8–16 biosensors); <8 pm (32–96 biosensors)
Baseline drift­­­
< 0.1 nm/hour
Quantitation range
32–96 biosensors: 0.1–100 μg/mL of hIgG at 1000 rpm**; 1.0–700 μg/mL at 400 rpm 8–16 biosensors: 0.05–300 μg/mL of hIgG at 1000 rpm**; 0.5–2000 μg/mL at 400 rpm
Quantitation precision
CV <10%
200 lbs (90.7 kg)
Dimensions (H x W x D)
30.1 in x 31.5 in x 31.5 in (77 cm x 80 cm x 80 cm)
Sample type
Proteins, antibodies, peptides, serum containing media (up to 25%), DMSO containing buffers, virus-like particles, untreated cell culture supernatants and crude cell lysates
Sample plate
Standard, 96-well and 384-well, black, tilted bottom microplates
Molecular weight detection
>150 Da (8–16 biosensors), >5000 Da (32–96 biosensors)

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