Octet® R2 Protein Analysis System
High Performance, Label-free Kinetics Characterization
Octet® R2 Protein Analysis System: Grows With Your Biomolecule Characterization Needs
Point of Product
Benefits
Samples are loaded in standard microplates, eliminating fluidic line maintenance and expanding the number of samples that can be run in an assay. Pre-defined templates in Octet® BLI Discovery software streamline setup prior to running an assay and minimizes training needs. The software also offers users a range of parameters and metrics for analyzing acquired data in a powerful yet intuitive software interface.
The 2-channel Octet® R2 system directly measures the presence of specific proteins and other molecules in solution with minimal interference from complex matrices. Accurate and reproducible concentrations can be determined in as little as 2 minutes per sample using a simple, one-step assay.
The sample plate temperature can be controlled from 15–40°C, which enables reliable kinetic determinations at different temperatures for temperature-sensitive proteins. Additional advantages of sample cooling include the ability to rapidly determine binding rate constants at multiple temperatures to extrapolate thermodynamic measurements. The Octet® R2 system’s two channels can be used independently to measure samples for screening purposes or in tandem, pairing the sample read with a dedicated reference for high-quality kinetic characterization.
4. Biosensor Re-Racking :
The Octet® R2 system provides the ability to re-rack biosensors at the end of an experiment. Biosensor re-racking provides enhanced flexibility in loading ligands on biosensors and reduces operational costs when biosensors are loaded with precious target reagents
5. Easy to Use :
Robust technology with simple, integrated user interface designed for biologists. Spend less time learning instrumentation and more time deriving insights.
Applications
Large Molecule Characterization
With pre-defined templates to follow in the Octet® BLI Discovery software, the Octet® R2 system streamlines setup prior to running an assay and minimizes training needs. Octet® Analysis Studio then gives users a range of parameters and metrics for analyzing acquired data, in a powerful yet intuitive software interface.
Small Molecule Characterization
With sensitivity to screen small molecule fragment libraries, the Octet® R2 instrument enables the measurement of interactions between small molecules down to 150 Da in size to determine accurate kinetics rates.
Specification
Detection Technology
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Bio-Layer Interferometry (BLI) based on fiber optic biosensors |
Biosensor Type
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Disposable, single-use fiber optic biosensors with optional reuse by regeneration and/or re-racking in the biosensor tray |
Data Presentation
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Graphs displaying real-time kinetic binding sensorgrams, fitted result plots, and residuals of fits Concentration data analysis including calibration curves and output of tabulated concentration data Epitope binning and cross-blocking matrices and trace overlays Tabulated kinetic data |
Number of Spectrometers
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2 |
Maximum Simultaneous Reads
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2 |
Data Collection Rate
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2, 5 or 10 Hz
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Sample Position and Format
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1 standard 96-well, black, flat-bottom microplate
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Sample Volume
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180–220 µL/well Nondestructive testing |
Orbital Flow Capacity
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Static or 400–1500 rpm
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Analysis Temperature Range
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15°C to 40°C, in 1°C increments
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Throughput
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Up to 2 assays in parallel
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Molecular Weight Detection
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>150 Da
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Analysis Time per Sample
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hIgG quantitation in 2 minutes for 2 samples Real-time kinetic binding experiments from 5 minutes to 4 hours for kinetic analysis |
Association Rate Constant (ka)
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101 to 107 M-1 s-1 |
Dissociation Rate Constant (kd)
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10-6 to 10-1 s-1 |
Affinity Constant (KD)
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1 mM – 10 pM
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Quantitation Range
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0.5 µg/mL to 2000 µg/mL (Human IgG and Protein A Biosensor) Protein - and assay-dependent |
Baseline Noise (RMS)
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<3.0 pm
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Baseline Drift
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< 0.1 nm/hour
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Quantitation Precision
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CV <10%
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Weight | 72 lb (32.7 Kg) |
Dimensions (H X W X D)
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19.5 in X 22 in X 18.2 in
(49 cm X 45 cm x 46 cm) |