1. High-Quality Kinetic Screening and Affinity Characterization :

The Octet® R8 system monitors binding events in real time to calculate on rates (ka), off rates (kd), and affinity constants (KD).

2. Versatility to Detect a Wide Range of Molecules

The high sensitivity Octet® R8 system detects a diverse range of biomolecules from small molecules to proteins to cells.

3. Eigh Parallel, Independent Channels for Maximum Speed and Flexibility

The Octet® R8 system’s eight channels can be used independently to measure samples for screening purposes or in tandem, pairing the sample read with a dedicated reference for high-quality kinetic characterization.

4. Perfecly Suited to Operate in GxP-Regulated Environments

Optional 21 CFR Part 11 software for Octet® R8 system available along with installation and qualification (IQOQ) and performance qualification (PQ) kits.

5. Sample Plate Cooling for Temperature-Sensitive Proteins

The sample plate temperature can be controlled from 15–40°C, enabling reliable kinetic determinations at different temperatures for temperature-sensitive proteins. Additional advantages of sample cooling include the ability to rapidly determine binding rate constants at multiple temperatures to extrapolate thermodynamic measurements. The Octet® R8 system’s eight channels can be used independently to measure samples for screening purposes or in tandem, pairing the sample read with a dedicated reference for high-quality kinetic characterization.

6. Fluidics-Free Dip and Read Format Reduces Assay Time and Maintenance Cost

The Octet® platform provides faster time to results and process economy when compared to other technologies due to its fluidics-free design. Additionally, crude sample compatibility saves valuable time by eliminating the need for sample pre-treatment, and non-destructive testing conserves precious samples for other assays.

Kinetic and Affinity Characterization

The superior sensitivity of the Octet® R8 system enables measurement of both small and large molecules and kinetic constants over a broad range. The system accurately measure kinetic constants by bringing the detection surface directly to the sample, eliminating the need for microfluidics. With ready to use biosensors for most proteins, the unique Dip and Read approach streamlines workflows and expedites assay development. It allows for direct measurement of crude samples with minimal instrument maintenance.

Protein Quantitation in Bioprocessing

Accurate and reproducible concentrations can be determined in as little as 2 minutes per sample or 32 minutes for a whole 96-well plate using a simple, one-step assay. High sensitivity in quantitation can be easily achieved by increasing read time and shake speed, and even higher sensitivity can be achieved to sub-ng/mL levels with 2-step or 3-step assay formats, allowing automated measurement of contaminants such as host cell proteins and residual protein A, faster and more precisely than ELISA. Process economics can be improved further by regenerating and reusing the biosensors.

Quality Control (QC) and Manufacturing

The Octet® R8 system has been developed to operate reliably in regulated quality control (QC) environments. The Octet® is aptly used in method development and validation for lot release and in-process testing for ligand binding, concentration, and impurity analysis in both upstream and downstream processes. The ease of use and simplicity in design together with 21 CFR Part 11 software and a full line of GxP products and services makes the Octet® R8 system an ideal analytical instrument for compliant labs.

Detection Technology	Bio-Layer Interferometry (BLI)
Biosensor Type	Disposable, single-use fiber optic biosensors with optional reuse by regeneration and/or re-racking in the biosensor tray
Data Presentation	Graphs displaying real-time kinetic binding sensorgrams, fitted result plots, and residuals of fits Concentration data analysis including calibration curves and output of tabulated concentration data Tabulated kinetic data Epitope binning and cross-blocking matrices and trace overlays
Sample Type	Proteins, antibodies, peptides, DNA, RNA, liposomes, viruses and VLPs in various media including serum, buffers containing DMSO, periplasmic fractions, untreated cell culture supernatants, and crude cell lysates
Number of Spectrometers	8
Maximum Simultaneous Reads	8
Data Collection Rate	2,5 or 10 Hz
Sample Position and Format	1 standard 96-well, black, flat-bottom microplate
Sample Volume	180–220 µL/well Nondestructive testing
Orbital Flow Capacity	Static or 100–1500 rpm
Analysis Temperature Range	15°C to 40°C, in 1°C increments

Quantitation

Workflow	Up to 8 assays in parallel
Analysis Time per Sample	Human IgG quantitation in 2 minutes for 8 samples, ≤ 32 minutes for 96 samples
DirectQquantitation Range for Human IgG with Protein A Biosensor	0.05 –2000 μg/mL

Weight	72 lb (32.7 Kg)
Dimensions (H x W x D)	19.5 in x 22 in x 18.2 in (49 cm x 56 cm x 46 cm)